Enhancement on antioxidant and antibacterial activities of Brightwell blueberry by extraction and purification

Abstract A blueberry anthocyanin extract was obtained from Brightwell fruits cultivated in eastern China and the extraction purification conditions were optimized. The components of identified using ultra-performance liquid chromatography-electrospray ionization interface-mass spectrometer. antioxidant antibacterial activities fruit supernatant (BFS), crude (BCE), rich (BRE) evaluated. yield 1.79 ± 0.0014 mg/g under following optimal conditions: 1:20 solid-to-liquid ratio (v/w), 24 h, 34 °C, 90% ethanol containing 0.21% (v/v) hydrochloric acid. With regard to purification, purity increased 19.1-fold. Nine fractions as glycosides delphinidin, cyanidin, petunidin, malvidin. biological improved through purification. Compared with BFS BCE, BRE had a higher DPPH radical scavenging activity (EC 50 = 0.51 mg/mL), ABTS capacity 0.32 oxygen absorbance (0.43 mmol Trolox/g). Furthermore, (2 mg/mL) showed maximum 84.64 0.35% reduction biofilm biomass Listeria monocytogenes inhibition zone given by against Escherichia coli 16.04 0.38 mm. highest capacities obvious effects food-related microorganisms than other samples. Therefore, can be used natural agent has potential health advantages food industry applications.